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V., Lai, M

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136 In addition, acquired astrocytopathies are prominent in hepatic encephalopathy (in which astrocytes lose their homoeostatic capabilities 137 ) and in neuromyelitis optica, in which the astrocyte protein, AQP4 is the subject of autoimmune attack, leading to dysfunction and death of astrocytes resulting in pronounced inflammation and degeneration of neural tissue

ROS-Glo HO Assay measures hydrogen peroxide levels in cell cultures using a sensitive, bioluminescent readout

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Figure 3 shows the synergistic positive feedback loop between ferroptosis and necroptosis, where ROS released during Erastin-induced ferroptosis triggers necroptosis, which in turn releases more ROS and iron to amplify the overall cell death signal