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About 500 ng of desalted peptide digests was separated on a 25 cm C18 column using a 1.5-h gradient elution and analyzed in a DIA mode in the Orbitrap Exploris 480 mass spectrometer (Thermo Fisher Scientific) equipped with a FAIMS unit

Glutathione is a compound found in nearly every cell and plays a role in maintaining cellular balance, supporting the bodys response to everyday environmental stressors, and promoting overall wellness

Interestingly, the effects of autophagy gene knockout differed based on the genetic background, especially regarding crucial tumor suppressors like APC and Tp53

Instead, asparagine supplementation enhances the expression of glutamine synthetase (GLUL) and increases intracellular glutamine usage via glutaminolysis, resulting in the recovery of global protein translation that is blocked by glutamine deprivation 45

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This leads to an impaired conversion of primary to secondary BAs, markedly shifting the overall BA composition