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Protein Isolation and Immunoblot Analyses Standard techniques were used as reported by us previously [29]

doi: 10.1016/j.nut.2009.04.005

A single amino acid has a general chemical formula: HN-CH(R)-COOH Here, the components are: HN-: Amino group (attached to the central carbon) -COOH: Carboxyl group (attached to the same central carbon) CH(R): The central carbon is bonded to a hydrogen atom (H) and a side chain group (R), which varies between different amino acids (e.g., R = -H for glycine, R = -CH for alanine)

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