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IF also reduces GH metabolic clearance and heightens lipolytic responsiveness, while attenuating IGF-I bioactivity and downregulating JAK/STAT signaling, as evidenced by decreased phospho-STAT5b/STAT5b ratios [22, 23]

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The beads were then washed twice, and bound protein was eluted with a 15-minute incubation in Elution Buffer

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doi: 10.3233/JAD-171110 64 HuangJ.ChenA.JinH.LiuF.HeiG.TengZ.et al