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10.1016/j.theriogenology.2024.04.013 169 WangJ.ZhangT.GuR.KeY.ZhangS.SuX.et al (2024b)

7 Tumor necrosis factor-alpha

Vegan formula, 60 capsules for a supply for 30 days

Stey C, Steurer J, Bachmann S, Medici TC, Tramr MR

Samples were reduced with DTT (5 L of 200 mM stock in H 2 O, 10 mM final concentration) and incubated at 65 C for 15 min, then alkylated with iodoacetamide (5 L of 400 mM stock in H 2 O, 20 mM final concentration) and shaken at 37 C for 30 min in the dark