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75 The most practical method of ensuring sufficient substrate is a 300 g marine fish meal followed by a random urine collection 212 hours after the meal or alternatively in the Nijmegen protocol, timed urine collection for 68 hours post meal

Ramos-HernndezRPascual-FernndezJMiguel-OrtegaMartnez-FerrnMFernndez-LzaroDBustoNet al

A major limitation in this field currently is a lack of animal and human experimental studies that validate in vitro and in vivo cell line and PDX work

Wild-type IDH1 overexpression fully rescued the phenotype, demonstrating that the observations are not due to off-target effects (Extended Data Fig

doi:10.1016/S0140-6736(20)32667-2

Statistical methods Variables were reported as percentages or means SD as appropriate