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Approaches such as chromatin immunoprecipitation have provided insight into direct targets of other pro-longevity transcription factors such as DAF-16, as well as its regulatory relationship with other transcription factors ( In vitro approaches to identify NHR-49 binding sites could represent a suitable avenue, as done for DAF-12 ( in vivo validation

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Yet, this is not true for all cases, since the XPF/ERCC1 heterodimer levels were increased in the most sensitive cell line and no difference in DNA repair capacity was detected comparing the resistant and sensitive tumor cell lines

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