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This reduction may occur through the decreased release of protein-bound vitamin B12 or by creating an environment with elevated pH that fosters intestinal bacterial overgrowth, subsequently diminishing vitamin B12 absorption [45,49]

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The in vitro MNT has gained widespread international regulatory acceptance (OECD Test Guideline 487), and is now recommended in several authoritative documents to be used in a core two test battery together with the Ames test (Kirkland et al., 201

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doi: 10.1016/j.jep.2016.10.003 366 Daz-de-CerioEVerardoVGmez-CaravacaAMFernndez-GutirrezASegura-CarreteroA