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Multiple sequence alignment for epitope conservation was performed using Clustal Omega 65 and conservation score was calculated as described in refs

For the assay, 1.5 mL of the protein extract was mixed with 5 mL of Bradford dye reagent (0.01% Coomassie Brilliant Blue G-250 dissolved in 4.7% ethanol and 8.5% phosphoric acid)

doi: 10.1016/j.jconrel.2014.02.002 204 PflanznerT.KuhlmannC

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5G), suggesting that Sirt7 directly interacts with nucleosomes via the C-terminal basic region

However, caution is advised when combining hesperidin with certain medications, and caution is advised to prevent diminished efficacy or enhanced adverse effects