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On a fellowship at Johns Hopkins University, Dorothy initiated her PhD program

Briefly, female BALB/c mice (6 weeks old) were fasted for 12 h, and a small amount of sterile medical-grade lubricant was applied to the anal region prior to rectal administration to facilitate insertion and minimize mechanical irritation

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65,66,67 Although the risk factors for PDPN are poorly understood, female sex, longer diabetes duration, increased neuropathy severity, renal dysfunction, higher HbA1c levels, and higher BMI have been suggested as contributing factors

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