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To validate this result, an alternative strategy to disrupt the pbgst gene was employed using a pbgst-ko plasmid with the hdhfr/yfcu positive-negative selectable marker (Figure 1E)

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PubMed Europe PMC Scholia Crystal structures of gamma-glutamyltranspeptidase from Escherichia coli, a key enzyme in glutathione metabolism, and its reaction intermediate

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A31572, Thermo Fisher Scientific)