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Subculture cells once the culture vessel is 80% confluent

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Among others, ageing is followed by a progressive reduction in liver volume and blood flow, parameters that drastically affect hepatic drug metabolism

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Based on our structural model, we hypothesized that proteolytic cleavage of this loop would lead to increased structural plasticity, particularly for residues 301307, which may, in turn, facilitate its induced-fit binding to HGF