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Mitochondriaplasma membrane interactions and communication

doi: 10.1016/j.canlet.2008.12.017

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For metabolite extraction, 100 L of plasma was combined with 400 L of cold extraction solvent (acetonitrile/methanol, 1/1, v/v) supplemented with 0.02 mg/mL L-2-chlorophenylalanine as an internal standard, and the samples were briefly mixed and subjected to low-temperature ultrasonication at 5 C for 30 min (40 kHz)