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10.1016/j.bbi.2013.02.005 52 ConnorJ

Standard cycle: 46 weeks Extended cycle with monitoring: up to 12 weeks for complex or chronic presentations Rest period between cycles: 48 weeks is typical Duration matters because tissue remodeling continues for weeks after a cycle ends

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Cell lysis was performed by immersing the slides in lysis buffer (2.5 M NaCl, 100 mM Na 2 EDTA, 10 mM Tris Base, 1% Triton X-100, 10% DMSO, pH 10) at 4 C for 1 h or overnight