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Protein expression was detected using an enhanced chemiluminescence (ECL) kit (NCM Biotech, Suzhou, China) and a ChemiDoc XRS System (Bio-Rad, CA, USA)

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In the USA alone, there were 608,366 cancer deaths in 2022, and 1,777,566 people were diagnosed with cases of invasive cancer alone in 2021, with medical expenses, both direct and indirect, projected to be around 146 billion USD [3,4,5,6]

MolProbity implemented in Phenix was used for model validation 44

Dysregulation of this pathway has been implicated in a myriad of diseases such as cancers, neurodegenerative diseases, and metabolic disorders, making the IGF-1 signaling pathway a prime target to develop therapeutic and intervention strategies

Prusty, like Naviaux, believes the mitochondria are much more than the energy producers of our cells they also play a key role in the cells antiviral response