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The control group showed higher abundances of Bacteroidota and Actinomycetota, while the VPA-induced ASD group exhibited increased Firmicutes and reduced Bacteroidota and Actinomycetota (Figure S5A)

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Laser lines and filters were set up for the appropriate channels as described in Supplementary Table 7

58,59 Works that reflect the antimicrobial activity of essential oils Essential oil of Origanum vulgare L found strong antimicrobial action against Staphylococcus aureus , Enterococcus faecium and, Escherichia coli isolates when tested for minimum inhibitory concentration, ranging from 0.29 to 1.15 mg/mL probably due to distraction of bacterial cytoplasmic membrane structure and function

Mice were euthanized three-and seven-weeks post infection for juvenile and adult worms, respectively, using a lethal dose of 0.018 ml of Euthasol and 5.85 mg/ml heparin to prevent blood coagulation (injection volume of 400 l)

Similarly, the methanolic leaf extract of papaya did not show acute toxicity at a dose of 2,000 mg/kg, and no sub-acute toxicity was observed after 28 days at the same dose