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General handling concepts sterile technique, site rotation, timing are covered here as general information

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To favor the recombination event and recovery of recombinants, the transformation mixtures were incubated overnight in liquid medium without antibiotic and in the presence of AdoCbl, since MetH requires B12 as cofactor

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Naturally occurring in the body, they play a vital role in processes like hormone production, immune response, cell communication, and tissue repair