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In order to assess the relationship between mannose release and the enzymatic degradation of the cell wall, we determined the activity of enzymes involved in the autolysis of yeast (protease and -glucosidase) in the media containing the untreated and PEF-treated biomass (Figure 7)

O'Daniel J, Dong L, Zhang L, de Crevoisier R, Wang H, Lee A, Cheung R, Tucker S, Kudchadker R, Bonnen M, Cox J, Mohan R , Kuban D

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For monoclonal antibodies, appropriate animals will be selected and the desired immunization protocol followed

The IC 50 concentrations of both compounds were dosed followed by successive dilutions, aiming to identify the doses at which the two drugs yield the IC 50 concentration