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Thus, requests for lower LODs and LOQs are well justified, and the evaluation of analytical methods strongly depends on these values

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For analysing the cell cycle profile, cells were incubated with 10 g/ml -1 BrdU for 1 hr, followed by cell surface staining, fixation and permeabilization based on the Phase-Flow Alexa Fluor 647 BrdU Kit (BioLegend)