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Alternatives to Ethanol based Water Solutions Ethylene glycol based water solutions Propylene glycol based water solutions Methanol based water solutions Example - Ethanol Concentration at Freezing Point at -20 o C By using linear interpolation between two known concentrations and their freezing points - the concentration at a third freezing point can be calculated as C C = ((C B - C A ) / (t B - t A )) (t C - t A ) + C A (1) where C = concentration in ethanol - water solution t = freezing point ( o C, o F) A, B = known freezing points C = calculated freezing point The ethanol concentration with freezing point at -20 o C can be calculated by interpolating the concentration between freezing point -15 o C and -23 o C in the table above

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The Model 3 solver function optimized three flux ratios to minimize the difference (i.e., error) between the predicted and measured 13 C lactate labeling pattern

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