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For the GSH estimation, 100 L of the supernatant was mixed with 900 L of reaction buffer (0.1 M phosphate buffer, pH 7.5, containing 5 mM EDTA), and the absorbance was measured at 412 nm using a spectrophotometer as the blank value 20

CoQ 10 oxidoreductases in the regulation of ferroptosis (1) FSP1 in the regulation of ferroptosis FSP1 as a therapeutic target in cancer FSP1 (formerly AIFM2) was first identified as a ferroptosis regulator among CoQ 10 oxidoreductases through independent unbiased genetic screens conducted by the Conrad and Olzmann groups under conditions of GPX4 inhibition 16,17

H: Histidine

104 WangJ

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217 TMM is expected to have better enzymatic performance over the Ruegeria pomeroyi enzyme, so we were sure to include this in our experimentation [7]