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This escalated oxidant milieu wreaks havoc at the cellular level in cardiac tissues, damaging proteins, lipids, and nucleic acids, culminating in severe endothelial dysfunction, which is especially detrimental in the context of heart health 48,49

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For each condition, approximately 30,000 cells were used per replicate in a total volume of 50 L, and an equal volume of CellTiter-Glo reagent was added in a 96-well plate

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