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Gong D, Lei J, He X, Hao J, Zhang F, Huang X, et al

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ACTL6A impacts GSH de novo synthesis mainly by upregulating -glutamyl-cysteine synthesis To further assess the mechanism by which ACTL6A regulates GSH metabolism, we investigated the metabolic fate of U- 13 C glucose by liquid chromatography with mass spectrometry (LCMS), which produces -GC containing two 13 C atoms via GSH de novo synthesis pathway, GSH containing two 13 C atoms via GSH de novo synthesis or the SGOC pathway, or four 13 C atoms via both pathways (Fig

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After 6 h, cell viability was measured with CellTiter-Blue , following the instructions of the manufacturer (Promega)

The tripeptide sequence glycyl-L-histidyl-L-lysine wraps around a copper ion, creating a structure with remarkable stability when handled correctly and surprising fragility when handled wrong