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Finally, add 50L of stop solution to each well and read the absorbance (OD value) at 450 nm using a microplate reader

doi: 10.1074/jbc.M508780200 265 WahrleS

The CYP2E1 induced increases oxidative stress in the hepatocytes, activates fibrotic signals by enhancing proliferation of stellate cells and increases collagen type 1 protein [83]

This robust defense systemworks well for us when were healthy, but in disease states, our defense against oxidants is weakened and the oxidant load increases to deleterious levels

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