l theanine and acetyl l carnitine Acetyl-L-carnitine – Cellular energy – Life Extension Europe Palmitoylethanolamide (OptiPEA"), Lecithin, L-Theanine, Alpha

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Alexa-Fluor 680 goat-anti-mouse IgG and IRDye 800CW goat-anti-rabbit IgG were used for detection

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Under the optimized experimental conditions, keeping the acquisition method and average times constant, the enhancement factor was calculated to be 128 with the intensity at the characteristic absorption peak of 1,654 cm 1

Overall, five studies were defined as high quality (Reference Hassan, Alalfy and Hassan18,Reference Badawy, El Nashar and El Totongy20,Reference El Sharkwy and Abd El Aziz24,Reference Kose and Naziroglu26,Reference Ghomian, Khadem and Moeindarbari5)

All data are expressed as the mean SD from at least three biological replicates, and comparisons between the two groups were performed with a nonparametric method (MannWhitney U test)