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Removing the first three N-terminal residues (Gly-Pro-Glu) lowers binding to IGF binding proteins, and in the research literature DES(1-3) IGF-1 is reported to bind the IGF-1 receptor with higher intrinsic affinity than the native sequence

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Since Zip14 KO did not completely inhibit efflux, other transporters, such as ZIP8, may also contribute, though in vivo studies are needed to confirm this

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