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To investigate the consequences of individual SBBYSS mutations on KAT6B transcript abundance and histone acetylation, we used CRISPR/Cas9 genome editing and homology-directed repair to generate clonal HEK293T cell lines carrying specific SBBYSS mutations spanning the KAT6B gene, validated by sequencing (Figure 1A and Supplemental Tables 1 and 2

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Depressive episodes are defined by decreased energy, fatigue, pervasive sadness, suicidal thoughts, and cognitive difficulties

doi: 10.32598/ppj.24.4.20 68

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