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Diabetes status was confirmed if fasting glucose was 7.0 mmol/l on two separate days or 7.0 mmol/l with hyperglycaemic symptoms or random glucose was 11.0 mmol/l, based on the diagnostic guidelines from the Dutch General Practitioners Association [21]

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The pathological features of IDD mainly include a reduction in the number of nucleus pulposus cells (NPCs), deterioration of the extracellular matrix (ECM), metabolic imbalance, and structural damage to the intervertebral disc (Vergroesen et al., 2015

Method validation All validation items were adequately assessed to guarantee that the techniques met ICH demands 37

Unlike the male hormonal system, the female endocrine system responds far more sensitively to exogenous androgens