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Janssens, H

doi: 10.1016/j.freeradbiomed.2013.09.015

Lipid analysis of epidermal and dermal cell membranes showed that they contain twelve types of these compounds, including phospholipids, such as phosphatidic acid (PA), phosphatidylethanolamines (PE), cardiolipins (CL), phosphatidylserine (PS), lysophosphatidylcholines (LPC), phosphatidylinositols (PI), alkylacylglycerophosphocholines (AAPC), plasmalogen ethanolamines (Epla), and phosphatidylcholines (PC), as well as sphingolipids (SP), such as dihydrosphingomyelins (DHSM), sphingomyelins (SM), and ceramides (Cer) [13, 35]

Cells were washed twice with PBS and treated with 30 g ribonuclease A and 15 g of propidium iodide

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Moreover, folic acid must be metabolized by dihydrofolate reductase (DHFR) to enter the folate cycle, with this reaction being slow and easily reaching saturation, thus limiting the therapeutic use of high doses of folic acid (Menezo et al ., 2022