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Firstly, the sample size included in this work is relatively small, and further validation and research with a larger sample size will be needed

Quick and minimally invasive with no surgery required

A change in pH has a negative effect on larval health and early development in Fishes (Kikkawa et al., 2003

, [45]

Academic and public health sources stress that while risks are generally low in trained hands, complications can occur if preparation or technique is poor

Potential metabolites were reported if they appeared in three or more time point samples, or if they appeared in a single sample but were part of a documented multi-transformation pathway