affinity purification of a glutathione-s-transferase fusion protein Schematic outline the GST-His two-step method Protein Purification by Affinity Chromatography

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Thereafter, trypsin at 1:50 was poured for overnight digestion and that at 1:100 was introduced for additional 4 h of digestion, followed by peptide desalting using a C18 SPE column

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Wohleb ES, Franklin T, Iwata M, Duman RS (2016)

Hospital of Cook County, (4)Baton Rouge General Medical Center, Baton Rouge, LA, (5)St Peter University Hospital, New Brunswick, NJ, (6)University of Ilorin Background: Portal vein thrombosis (PVT) is associated with gastrointestinal malignancies such as hepatocellular, biliary, pancreatic, and colonic cancers

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