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Van Breusegem F (2006) Reactive oxygen species in Plant Cell Death

RoseRASellanMSimpsonJAIzaddoustdarFCifelliCPanamaBKet alIron overload decreases Cav1.3-dependent L-type Ca2+ currents leading to bradycardia, altered electrical conduction, and atrial fibrillation

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Mild : Tachycardia, Hypertension, Nausea, Sweating Severe : Headache, Palpitations, Neck Stiffness, Photophobia, Chest pain Life Threatening : MI, Stroke, Pulmonary Edema Onset 30-60 mins after ingestion

For fluorescence staining, we incubated samples for 2 h with Alexa-488 and Alexa-594 conjugated donkey antibodies (1:200) against mouse and rabbit immunoglobulins (Supplementary Figs 6 and 7 are representative images from two sets of immunolabelled cultures of NPCs and neurons)