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Characterization of the isoenzymes pyruvate dehydrogenase phosphate implications for the regulations of pyruvate dehydrogenase activity

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This was achieved by maximizing the activity of reactions that are associated with upregulated genes and minimizing flux through reactions that are downregulated in a condition, while simultaneously satisfying the stoichiometric and thermodynamic constraints embedded in the model using linear optimization 44,81

However, this synergistic effect does not appear to be strictly required for the reaction to occur

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