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After 4 hours, nonadherent cells were removed, and the culture medium was replaced with Lonza HCM hepatocyte medium (CC-3198) or continued in DMEM with 20% FBS, depending on experimental needs

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Consequently, we decided not to include Rosetta-based calculations in this study and relied on FoldX calculations, using 25 representative structures from each of the MD replicates of the constructs LC3B 1-120 -OPTN 169-185 and LC3B 1-120 -OPTN 158-191 from Table S1