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Briefly, cells on coverslips were fixed with 4% paraformaldehyde for 15 min at 25 C

Blas-Valdivia V, Rojas-Franco P, Serrano-Contreras JI et al (2021) C-phycoerythrin from Phormidium persicinum prevents acute kidney injury by attenuating oxidative and endoplasmic reticulum stress

98 In addition, Muc2 gene mutation in mice can induce endoplasmic reticulum stress in intestinal epithelial cells, and the misfolding of intrinsic proteins in the intestinal epithelium triggers the inflammatory response of intestinal epithelial cells, thereby destroying the structure and function of the intestinal barrier in mice and promoting the progression of UC by affecting the intestinal microbiota

doi:10.1186/1477-044X-1-2

The usual frequency of IVs is twice weekly

Short-term calorie restriction reverses vascular endothelial dysfunction in old mice by increasing nitric oxide and reducing oxidative stress