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Structural characterization of purified mosaic capsid particles was performed with ELISA, native agarose gel electrophoresis, pull-down assay, electron microscopy and NMR spectroscopy

Louis, MO, USA) was injected into the trachea to induce Silicosis model, and 9% normal saline solution was injected into the sham group

Use 5-10 bed volume of Elution buffer (added GSH) to elute, collect in fractions, collect one tube for each bed volume, and test separately, which can not only ensure that all binding target proteins are eluted, but also obtain high-purity and high-concentration proteins

ShD14 was able to hydrolyze rac -GR24 and 5DS but not YLG, suggesting that ShD14 might also serve as an SL receptor