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Since enzymes are present in blood circulation, the team checked out the deterioration of the nanofibres susceptible to enzyme hydrolysis utilizing peptidases

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After those membranes were washed with TBS for one hour and then incubated with secondary antibody-HRP using a dilution of 1:7000 in TBS for 40 min, then washed twice with Tween-TBS for 15 min followed by twice washing with TBS for 15 min each

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