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100 l were used for perifusion for each group using a PERI4 machine (Biorep Technologies, Miami, FL, USA), allowing to run all three conditions in parallel

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Afterward, samples were dehydrated through a graded alcohol series then paraffin-embedded, sectioned (5 m thickness), and stained with hematoxylin and eosin (H&E) to illustrate the general histological components with subsequent examination conducted under a light microscope (BX51, Olympus, Tokyo, Japan), and photomicrographs were acquired at 10X and 40X objectives (Paul and Chanda 2017)