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Tissues were then incubated in 10, 20, and 30% sucrose in 1 PBS, embedded in OCT compound (Tissue-Tek, Sakura Finetek, 4583), snap frozen on dry ice, and then stored frozen at 80C

Conclusion: Donor inflammatory burden was increased relative to non-critically ill patients, indicating that donor inflammation may play a role in long term DCD graft dysfunction

Ascorbic acid is present at high levels in all parts of plants and can reach concentrations of 20 millimolar in chloroplasts

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Glutamine is formed by the incorporation of a second ammonium ion into glutamate by the help of the enzyme glutamine synthase

Biochem J 360:116 Shi H, Jiang C, Ye T, Tan DX, Reiter RJ, Zhang H, Liu R, Chan Z (2015) Comparative physiological, metabolomic, and transcriptomic analyses reveal mechanisms of improved abiotic stress resistance in bermudagrass [Cynodon dactylon (L.) Pers.] by exogenous melatonin