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Glucoraphanin is the major glucosinolate associated with many health-benefits, as well as the prebiotic effects in broccoli

S-containing metabolites, including amino acids methionine, major reductant glutathione, and specific metabolites, are synthesized from cysteine

To identify which pathway contributes most to QEC tolerance to Cys-Cys limitation, we treated QECs with specific inhibitors targeting STAT3 and NRF2 activity: AG-490 [40] and ML385 [41], respectively

After 48 h of incubation, the biofilms were washed once with 1 PBS, followed by addition of 200 L of 1 PBS and 15 L of a 0.05% w/v resazurin solution

Glutathione is generally well-tolerated by most people because it is a substance the body already knows how to process

At each time point, 3 mice were randomly selected for sampling