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More important than concentration is the formulation's pH stability (GHK-Cu is stable between pH 5.0 and 6.5) and the carrier's ability to deliver the peptide past the skin barrier

The complete approval process consists of five major stages: preclinical toxicology studies, Phase I human safety and tolerance testing, Phase II efficacy evaluation, Phase III large-scale controlled clinical trials, and GMP manufacturing site audits

Experiments are designed to quantify its effects on collagen I and III synthesis (via qPCR or Western blot), myofibroblast differentiation (by measuring -smooth muscle actin expression), and the critical MMP/TIMP balance

measured MMP1, MMP2, TIMP1, and TIMP2 gene expression in human adult dermal fibroblasts exposed to GHK-Cu at 0.01, 1, and 100 nanomolar concentrations and found dose-dependent increases in both MMPs and their inhibitors 11