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We attempted to overcome these limitations by focusing on a single pathway, using an information-based gene-selection algorithm (based on location in autism linkage regions and expression patterns), selecting tag-single-nucleotide polymorphisms (SNPs) to achieve very high coverage of genes and flanking regions, and by evaluating high order genegene interactions using a newly developed method adapted for family data

The GABA/glutamate balance can also be affected by autoimmunity and genetic disorders

Cutting edge: NF-kappaB activating pattern recognition and cytokine receptors license NLRP3 inflammasome activation by regulating NLRP3 expression

In addition, it also acts as an acetyl storage, providing energy in carbohydrate and lipid metabolic pathways [48]

RT-PCR was performed using LightCycler 480 II Real-time PCR Instrument (Roche, Basel, Switzerland) with QuantiFast SYBR Green qPCR Master Mix (Qiagen, Dsseldorf, Germany)

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