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Before analysis, cells were washed once with PBS, incubated in 175 l XF base media (Agilent Technologies) supplemented with 2 mM glutamine with or without 100 nM insulin, and incubated at 37C without CO 2 for 1 hour

BPC-157 rapidly activates collateral blood vessels to bypass vascular obstructions, as demonstrated in colonic ischemia models where the peptide restored blood flow around ligated vessels within minutes to hours of administration

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Preclinical research demonstrated that SS31 protects against mitochondrial dysfunction across multiple disease models including heart failure, ischemiareperfusion injury, neurodegeneration, chronic kidney disease, and agerelated muscle atrophy [9]