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Based on this finding, the research team proposed a new strategy for inhibiting the glutathione metabolic pathway to treat IDH1 mutant glioma

Kimmel PLSS, Patel

At 8 DIV, growth medium was replaced with defined serum-free culture medium consisting of a mixture of a buffered saline solution (10 mM HEPES, pH 7.4, 114 mM NaCl, 26.1 mM NaHCO 3 , 5.3 mM KCl, 1 mM MgCl 2 , 2 mM CaCl 2 , 5 mM glucose, 1 mM glycine, 0.5 mM C 3 H 3 NaO 3 , and 0.001% phenol red) and phosphate-free Eagle's minimum essential medium (9:1 v/v) supplemented with insulin (7.5 g/mL), transferrin (7.5 g/mL), and sodium selenite (7.5 ng/mL) (ITS supplement, Merck Millipore, #I3146)

Additionally, glutathione, an essential tripeptide antioxidant, is vital for GPX4's function in neutralizing hydrogen peroxide [13,14,15]

Touati et al

Dermatol 24 (2), 155157