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Frozen cells were dounce-homogenized in cold homogenization buffer (20 mm Tris-HCl pH 7.4), containing EDTA-free protease inhibitor cocktail (Roche) and PhosSTOP phosphatase inhibitor cocktail (Roche)

As NADPH is crucial for antioxidant pathways, such as glutathione and thioredoxin, ME1 upregulation suggests enhanced cellular capacity for ROS

doi: 10.1111/nan.12149 158 BoehlerJFHornANovakJSLiNGhimbovschiSLundbergIEet al

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