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Delivery approaches for CRISPR/Cas9 therapeutics in vivo: advances and challenges

10.1016/j.neulet.2012.01.066 Neurosci

Y.et al (2017)

It has been shown that supplementation of a diet with lutein or zeaxanthin improves contrast sensitivity and visual functions by stimulating neuronal signaling activity in the eye (Stringham and Hammond 2005)

Y.LuY.SunX

At 48 h post-transfection, cells were harvested in ice-cold PBS using Trypsin-EDTA (Lonza, Switzerland), centrifuged at 1000 g for 3 min, and washed with ice-cold PBS