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MS analysis Peptides were re-dissolved in 20 l loading solvent A [0.1% trifluoroacetic acid in water/acetonitrile (ACN) (99.5:0.5, v/v)] of which 2 l of sample was injected for LC-MS/MS analysis on an Ultimate 3000 ProFlow nanoLC system in-line connected to a Q Exactive HF mass spectrometer (Thermo) equipped with pneu-Nimbus dual ion source (Phoenix S&T)

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2.0.CO;2 Xu Y, Ye H, Ma J, Huang H, Wang G (2010) Primary culture and characteristic morphologies of neurons from the cerebral ganglion of the mud crab, Scylla paramamosain