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An outer region of interest was drawn for every lumen by drawing a rectangular region approximately spanning from the midpoint of the diagonal of the top half of the ECM barrier, down to the lumen edge and across to the other side of the ECM barrier so that the edges of the device are not included in the outer region of interest

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Kalt et al., 2020)

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All other data, including immunoblots, GSH and AA, were also analysed for statistical significance using a two-tailed unpaired Students t -test unless stated otherwise