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ZEA and its derivatives produce estrogenic effects in various animal species The structure of the ZEA similar to steroids and binds to ER as an agonist

Immunofluorescence After PBS washing (twice), cells on coverslips were fixed with 4% paraformaldehyde (20 min), permeabilized with 0.1% Triton X-100 (room temperature), and blocked with 3% BSA (30 min, RT)

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