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Biochem J 434:201210

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Immunohistochemistry Anesthesia was induced with 5% isoflurane in an induction box and maintained with 1.5% isoflurane via a face mask throughout the operation

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Circadian clock and immunity Almost all the components of the immune system, involved in adaptive and innate immunity, have been observed to exhibit circadian variations (for a comprehensive review on the topic see 50,51 For example, rhythmic oscillations occur in the trafficking of hematopoietic stem cells and leukocytes, in the expression of recognition receptors and their downstream signaling pathways, and finally in the production of cytokines and chemokines